Discovery and Characteristics of a Novel Antitumor Cyclopeptide Derived from Shark

Discovery and Characteristics of a Novel Antitumor Cyclopeptide Derived from Shark

Blog Article

Peptides pose a challenge in drug development due to their short half-lives in vivo.In this study, we conducted in vitro degradation experiments on SAIF, which is a shark-derived peptide that we Shorts previously studied.The degradation fragments were sequenced and a truncated peptide sequence was identified.The truncated peptide was then cloned and expressed via the E.

coli system with traceless cloning to form a novel cyclic peptide in vitro oxidation condition via the formation of a disulfide bond between the N- and C-termini, which Jumpsuits was named ctSAIF.ctSAIF exhibited high anti-HCC activity and enhanced enzymatic stability in vitro, and retained antitumor activity and good biocompatibility in systemic circulation in a HCC xenograft model.Our study discovered and characterized a novel shark-derived cyclic peptide with antitumor activity, laying a foundation for its further development as an antitumor drug candidate.The study also provided a new solution for peptide drug development.